2. Author's Information
    G Roughan
    The Horticulture and Food Research Institute of New Zealand Ltd., Mt. Albert Research Centre, Private Bag 92169, Auckland, New Zealand
  3. Abstract
    A semi-preparative enzymic synthesis of [1-14C]malonyl-CoA from [1-14C]acetate and bicarbonate, and of [3-14C]malonyl-CoA from Na2(14)CO3 and acetate, was achieved by using chloroplasts rapidly isolated from 7-8-day-old pea shoots. Around 70% of the [1-14C]acetate was converted into malonyl-CoA in 2-3 h, and the specific radioactivity of [3-14C]malonyl-CoA synthesized in the system was 25-30 Ci/mol. Reactions were monitored and labelled products were purified by h.p.l.c.
    Keywords
    semi-preparative enzymic, malonyl-CoA, [14C]acetate
    DOI: 10.1042/bj3000355
    ADLID: 78955-v4
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  1. Keywords
    semi-preparative enzymic malonyl-CoA [14C]acetate
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