Sequence-Specific Binding of [N-MeCys3,N-MeCys7]TANDEM to TpA

Research Article | Biochemical Journal
Sequence-Specific Binding of [N-MeCys³,N-MeCys⁷]TANDEM to TpA
Author's Information

M Lavesa

Department of Physiology and Pharmacology, University of Southampton, Bassett Crescent East, Southampton, U.K.

R K Olsen

Deparment of Chemistry and Biochemistry, Utah State University, Logan, UT, 84322, U.S.A.

K R Fox

Department of Physiology and Pharmacology, University of Southampton, Bassett Crescent East, Southampton, U.K.
Abstract

The sequence selective binding of [N-MeCys3,N-MeCys7]TANDEM to DNA has been studied by footprinting experiments on DNA fragments containing the self-complementary sequences CGCGATATCGCG, CGCGTATACGCG, CGCGTTAACGCG and CGCGAATTCGCG. DNAase I and micrococcal nuclease reveal drug-induced footprints with the central sequences ATAT, TATA and TTAA, but not AATT, suggesting that the ligand binds to the dinucleotide TpA. The ligand renders certain adenines hyper-reactive to diethyl pyrocarbonate. These are observed with ATAT, TATA and TTAA, but not AATT, and are located both within, and distal to, the TpA-binding sites.

Keywords

Sequence-specific binding, TpA-binding sites, micrococcal nuclease

DOI: 10.1042/bj2890605

ADLID: 85357-v4

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Keywords
Sequence-specific binding TpA-binding sites micrococcal nuclease

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